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GHK-Cu also suppresses TGF-β1/SMAD-mediated fibrosis and activates Nrf2-ARE antioxidant response elements
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When we seeded Cas9-expressing HCT-8 cells (induced using 0.5 μg/mL doxycycline) along with transfection reagent Dharmafect 2 and 10 nM tracrRNA into wells containing 10 nM pooled guides against a particular gene, we found protein levels reduced by 70-80 % three days later (Fig S1D)
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